Dentro e fora da sala de aula: uma experiência pedagógica em Ciências Biomédicas Laboratoriais

O Bioempreendedorismo é associado às biotecnologias ou ciências da saúde sendo uma das unidades curriculares normalmente lecionada em cursos de Escola Superiores de Saúde do Ensino Superior Politécnico em Portugal. Estes cursos, têm em regra, quatro anos de duração, sendo lecionada a unidade curricular no 3º ou 4º ano, encontrando-se alguns dos alunos a frequentar os seus estágios curriculares. Neste artigo é efetuado um estudo exploratório onde são comparados os resultados da aprendizagem no curso de Ciências Biomédicas Laboratoriais da Escola Superior de Saúde Dr. Lopes Dias do Instituto Politécnico de C. Branco, em duas situações distintas: ensino dentro da sala de aula, na sua forma presencial tradicional e o ensino fora da sala de aula, sob a forma de ensino à distância. São utilizados como critérios de comparação as ideias de negócio desenvolvidas pelos alunos assim como também os resultados da avaliação. Neste artigo são apresentados os conteúdos programáticos, a metodologia de ensino e os meios utilizados em ambas as situações. No final são discutidos os resultados obtidos neste estudo exploratório que decorreu nos anos letivo de 2015/16 e 2016/17.info:eu-repo/semantics/publishedVersio

A Simple Approach to Detect Anomalies in Microservices-Based Systems Using PyOD

Ease of scale is one of the defining characteristics of microservices. However, with scalability comes the problem of diversity of services, making it very important to detect anomalies the soonest possible. Because it is recent, there are still few studies on the best approaches to detecting anomalies in microservices. This paper proposes the Python toolkit, PyOD, as an approach for microservice anomaly detection. This toolkit is composed of a set of anomaly detection algorithms, including classical LOF (SIGMOD2000) to the latest ECOD (TKDE2022). To evaluate the approach, we used two of its algorithms, k Nearest Neighbors (kNN) and Histogram-based Outlier Score (HBOS) to detect anomalies such as application bugs, CPU exhausted, and network jam on the TraceRCA dataset. This dataset contains logs from a real microservices system. The preliminary results show that HBOS algorithm performs better than kNN, with Recall and F1-Score of 93% and 89%, respectively, while for kNN these metrics were 92% and 85%, respectively

Projeto de instalações elétricas, telecomunicações e segurança contra incêndios de um Centro Escolar

No âmbito da obtenção do grau de mestre em Instalações e Equipamentos em Edifícios, do Instituto Superior de Engenharia de Coimbra, sobe a orientação do Doutor Paulo José Gameiro Pereirinha e Doutora Cristina Isabel Ferreira Figueiras Faustino Agreira, foram desenvolvidos os projetos das especialidades de Instalações Elétricas, Infraestruturas de Telecomunicações e Segurança Contra Incêndios de um centro escolar. Este trabalho tem como objectivo realizar o estudo das várias especialidades e dotar o edifício das infra-estruturas necessárias ao seu bom funcionamento, bem como, a comodidade e segurança dos seus utilizadores. O presente trabalho está dividido essencialmente em quatro partes, o projeto Elétrico, o projeto de Infra-estruturas de Telecomunicações, o projeto de Segurança Contra Incêndios e por último a parte de Medição e Orçamento. A elaboração de cada projeto de especialidade foi desenvolvida mediante três passos, o estudo prévio da legislação, normas e regulamentos em vigor, aplicadas ao tipo de edifício em questão, dimensionamento dos diversos equipamentos e infra-estruturas de cada especialidade resultante da aplicação prática da legislação vigente, e por último elaboração de peças desenhadas da distribuição dos diversos equipamentos pelo edifício e da documentação técnica

Osteoblasts and bone formation

© 2007 Sociedade Portuguesa de ReumatologiaBone is constantly being remodelled in a dynamic process where osteoblasts are responsible for bone formation and osteoclasts for its resorption. Osteoblasts are specialized mesenchymal cells that undergo a process of maturation where genes like core-binding factor α1 (Cbfa1) and osterix (Osx) play a very important role. Moreover, it was found recently that the Wnt/β-catenin pathway plays a part on osteoblast differentiation and proliferation. In fact, mutations on some of the proteins involved in this pathway, like the low-density lipoprotein receptor related protein 5/6 (LRP5/6), lead to bone diseases. Osteoblasts have also a role in the regulation of bone resorption through receptor activator of nuclear factor-κB (RANK) ligand (RANKL), that links to its receptor, RANK, on the surface of pre-osteoclast cells, inducing their differentiation and fusion. On the other hand, osteoblasts secrete a soluble decoy receptor (osteoprotegerin, OPG) that blocks RANK/RANKL interaction by binding to RANKL and, thus, prevents osteoclast differentiation and activation. Therefore, the balance between RANKL and OPG determines the formation and activity of osteoclasts. Another factor that influences bone mass is leptin, a hormone produced by adipocytes that have a dual effect. It can act through the central nervous system and diminish osteoblasts activity, or can have an osteogenic effect by binding directly to its receptors on the surface of osteoblast cells.info:eu-repo/semantics/publishedVersio

Blisstrail: An Agile Project Business Case Study

AbstractToday's software development environment dictates speed, flexibility and a people-centered focus. Virtual teams represent a new organizational form that does not provide for same proximity collaboration. In this paper we propose a solution approach of a virtual team, as well as show some examples of using the Microsoft Visual Team Foundation Server 2013 tools to address these challenges

The role of military leadership in the socio-professional configuration of Portuguese police administrative elites

This article aims to understand the relationship between the military and police universes, particularly in the PSP, a security force with a civilian matrix, but based on principles inherited from a military culture. In order to achieve this, it is crucial to highlight the creation of the Escola Superior de Polícia in 1982; the designation and curriculum of the Police officers training course, the 1999 Organic Law, which strengthened its civilian matrix; and rituals, categories, symbols, careers, graduations, parades, salutes and police honours. To accomplish this purpose we intend, with the contribution of a socio-historical appreciation, to answer the following question: what was the contribution of the military leadership to the consolidation of police administrative elites in Portugal? As specific objectives, we consequently delineated the following topics: understanding what it means to be a leader in the current Portuguese Police; identify the military-inspired characteristics of the Portuguese police; and comprehend if the leadership in the Police is distinguished from the action taken by a military leaderinfo:eu-repo/semantics/publishedVersio

Big Data and Traffic Data Analysis: Urban Traffic Forecasting and Management

The paper reviews different methodologies and technologies used in urban traffic management and analysis systems based on Big Data. Several techniques are discussed, such as the installation of sensors, IoT devices and surveillance cameras for data collection, the use of machine learning algorithms and technologies for vehicle identification and classification, the use of Map Reduce techniques for data processing, and the communication between several social network applications to obtain real-time data about the location and movement of users. In addition, models based on neural networks and algorithms such as ABC are proposed for signal change prediction and traffic flow management. The advantages and disadvantages of each approach and how they can be applied to improve urban traffic management and reduce vehicle pollution are highlighted

Igreja Católica e direito criminal: uma abordagem sociológica ao Código Penal Português (1886)

O protagonismo da religião nas sociedades modernas é uma matéria que faz parte dos debates fundamentais das Ciências Sociais, surgindo a Igreja Católica como um campo de análise com especial interesse para o estudo sociológico da mudança na sociedade portuguesa. Este artigo visa analisar a influência da Igreja Católica no Direito Criminal português, maxime o Código Penal (1886), também designado como Código Veiga Beirão. Metodologicamente, privilegiou-se uma abordagem histórico-sociológica na perspetiva de contribuir para o conhecimento sociológico da evolução do Direito Penal e a influência da Igreja Católica na construção do edifício jurídico-criminal nacional.The role of religion in modern societies is a matter that is part of the fundamental debates of the Social Sciences, and the Catholic Church emerges as a field of analysis with a particular interest in the sociological study of change in Portuguese society. This paper aims to analyze the influence of the Catholic Church in the Portuguese Criminal Code of 1886, also known as the Veiga Beirão Code. Methodologically we favored a historical-sociological approach from the perspective of contributing to the sociological knowledge of the evolution of criminal law and the influence of the Catholic Church in the construction of the Portuguese legal-criminal system.info:eu-repo/semantics/publishedVersio

Low Serum Levels of DKK2 Predict Incident Low-Impact Fracture in Older Women

There are currently no robust noninvasive markers of fragility fractures. Secreted frizzled related protein-1 (sFRP-1), dickkopf-related protein 1 (DKK1) and DKK2, and sclerostin (SOST) inhibit Wnt signaling and interfere with osteoblast-mediated bone formation. We evaluated associations of serum levels of sFRP-1, DKK1, DKK2, and SOST with incident low-impact fracture and BMD in 828 women aged ≥65 years from EpiDoC, a longitudinal population-based cohort. A structured questionnaire during a baseline clinical appointment assessed prevalent fragility fractures and clinical risk factors (CRFs) for fracture. Blood was collected to measure serum levels of bone turnover markers and Wnt regulators. Lumbar spine and hip BMD were determined by DXA scanning. Follow-up assessment was performed through a phone interview; incident fragility fracture was defined by any new self-reported low-impact fracture. Multivariate Cox proportional hazard models were used to analyze fracture risk adjusted for CRFs and BMD. During a mean follow-up of 2.3 ± 1.0 years, 62 low-impact fractures were sustained in 58 women. A low serum DKK2 level (per 1 SD decrease) was associated with a 1.5-fold increase in fracture risk independently of BMD and CRFs. Women in the two lowest DKK2 quartiles had a fracture incidence rate of 32 per 1000 person-years, whereas women in the two highest quartiles had 14 fragility fractures per 1000 person-years. A high serum sFRP1 level was associated with a 1.6-fold increase in fracture risk adjusted for CRFs, but not independently of BMD. Serum levels of SOST (r = 0.191; p = 0.0025) and DKK1(r = -0.1725; p = 0.011) were correlated with hip BMD, but not with incident fragility fracture. These results indicate that serum DKK2 and sFRP1 may predict low-impact fracture. The low number of incident fractures recorded is a limitation and serum levels of Wnt regulators should be further studied in other populations as potential noninvasive markers of fragility fractures. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.publishersversionpublishe

Celastrol efficacy by oral administration in the adjuvant-induced arthritis model

Copyright © 2020 Cascão, Vidal, Carvalho, Lopes, Romão, Goncalves, Moita and Fonseca. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Background: We previously demonstrated that celastrol has significant anti-inflammatory and bone protective effects when administered via the intraperitoneal route. For further preclinical evaluation, an effective oral administration of celastrol is crucial. Here we aimed to study the therapeutic dose range for its oral administration. Methods: Celastrol (1-25 μg/g/day, N = 5/group) was administrated orally to female adjuvant-induced arthritis (AIA) rats after 8 days of disease induction for a period of 14 days. A group of healthy (N = 8) and arthritic (N = 15) gender- and age-matched Wistar rats was used as controls. During the treatment period, the inflammatory score, ankle perimeter, and body weight were measured. At the end of the treatment, the animals were sacrificed, blood was collected for clinical pathology, necropsy was performed with collection of internal organs for histopathological analysis, and paw samples were used for disease scoring. Results: Doses higher than 2.5 μg/g/day of celastrol reduced the inflammatory score and ankle swelling, preserved joint structure, halted bone destruction, and diminished the number of synovial CD68+ macrophages. Bone resorption and turnover were also reduced at 5 and 7.5 μg/g/day doses. However, the dose of 7.5 μg/g/day was associated with thymic and liver lesions, and higher doses showed severe toxicity. Conclusion: Oral administration of celastrol above 2.5 μg/g/day ameliorates arthritis. This data supports and gives relevant information for the development of a preclinical test of celastrol in the setting of a chronic model of arthritis since rheumatoid arthritis is a long-term disease.info:eu-repo/semantics/publishedVersio